Antagonistic cytoprotective effects of C60 fullerene nanoparticles in simultaneous exposure to benzo[a]pyrene in a molluscan animal model

The hypothesis that C60 fullerene nanoparticles (C60) exert an antagonistic interactive effect on the toxicity of benzo[a]pyrene (BaP) has been supported by this investigation. Mussels were exposed to BaP (5, 50 & 100 ?g/L) and (C60–1 mg/L) separately in combination. Both shown co-localize secondary lysosomes hepatopancreatic digestive cells gland where they reduced lysosomal membrane stability (LMS) or increased permeability, while also induced lipid lipofuscin, indicative oxidative cell injury autophagic dysfunction. Combinations showed effects for stability, mTORC1 (mechanistic target rapamycin complex 1) inhibition intralysosomal (5 ?g/L BaP). biomarker data (i.e., LMS, lipidosis lipofuscin accumulation; lysosomal/cell volume dephosphorylation mTORC1) further analysed using multivariate statistics. Principal component cluster analysis clearly indicated its own was more injurious than combination with C60. Use a network model integrated pathophysiological processes, there significant interactions complexity (% connectance) at all concentrations combined treatments. Loss probably causes release iron hydrolases into cytosol, can generate harmful reactive oxygen species (ROS). It inferred adverse reaction ameliorated treatments ROS scavenging property C60, thus limiting membrane; reducing damage cytosol nuclear DNA. enhanced turnover damaged constituents, appeared have cytoprotective against toxic